rabbit polyclonal antibody against ki 67  (Proteintech)

Journal: Nature Communications

Article Title: Dimeric copper peptide incorporated hydrogel for promoting diabetic wound healing

doi: 10.1038/s41467-025-61141-1

Figure Lengend Snippet: a Representative fluorescence images of Ki67 staining of HUVEC cells after different treatments (green: Ki67; blue: cell nucleus, Scale bars = 20 µm). b Quantitative analysis of Ki67+ cells. Data represented as Mean ± SD (n = 3). Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. c Relative cell viability of NIH/3T3 cells co-cultured with D-CuP solution and M-CuP solution for 24 h. Data represented as Mean ± SD (n = 4 independent replicates, ns not significant). Statistical significance was assessed using two-way ANOVA with Tukey’s multiple comparisons test. d Representative images of tube formation assay in HUVEC cells stained with calcein-AM (green) (Tube: yellow arrow, Scale bars = 400 µm). e Quantitative analysis of total tube length. Data represented as Mean ± SD (n = 3). Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. f The concentrations of VEGF were determined by ELISA. Data represented as Mean ± SD (n = 3 independent replicates). Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. g Representative images of transwell migration assay of L929 cells (Scale bars = 200 µm). h Quantitative analysis of total tube length. Data represented as Mean ± SD (n = 3). Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. i Images of L929 cells scratch experiment after incubating with D-CuP or M-CuP at different times (Scale bars = 100 μm). j Cell scratch healing rates at different times. Data represented as Mean ± SD (n = 3 independent replicates). Statistical significance was assessed using two-way ANOVA with Tukey’s multiple comparisons test. Source data are provided as a Source Data file.

Article Snippet: Cells were incubated with rabbit polyclonal antibody against Ki67 (1:500, Cat No. 28074-1-AP, Lot No. 00141310, Proteintech, China), followed by CoraLite488 goat anti-Rabbit IgG(H + L) secondary antibody (1:500, Cat No. SA00013-2, Lot No. 20000839, Proteintech, China).

Techniques: Fluorescence, Staining, Cell Culture, Tube Formation Assay, Enzyme-linked Immunosorbent Assay, Transwell Migration Assay

Journal: Nature Communications

Article Title: Dimeric copper peptide incorporated hydrogel for promoting diabetic wound healing

doi: 10.1038/s41467-025-61141-1

Figure Lengend Snippet: The concentrations of ( a ) IL-6, ( b ) IL-10, and d VEGF in the wound tissue were determined by ELISA on day 3. Data represented as Mean ± SD. (n = 3 independent replicates) Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. c Representative images of immunohistochemistry staining of TNF-α, VEGF, CD31, and Ki67 (Scale bars = 100 μm) on day 7 and Masson staining on day 12 (Scale bars = 500 μm). Quantitative analysis of immunohistochemical staining for ( e ) CD31 and ( f ) Ki67 on day 7. Data represented as Mean ± SD. (n = 3 independent replicates) Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. g Quantification of the collagen deposition in different groups on day 12. Data represented as Mean ± SD (n = 3 independent replicates). Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. Source data are provided as a Source Data file.

Article Snippet: Cells were incubated with rabbit polyclonal antibody against Ki67 (1:500, Cat No. 28074-1-AP, Lot No. 00141310, Proteintech, China), followed by CoraLite488 goat anti-Rabbit IgG(H + L) secondary antibody (1:500, Cat No. SA00013-2, Lot No. 20000839, Proteintech, China).

Techniques: Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Staining, Immunohistochemical staining